Set 4 water bath/blocks: 60C, 55C, 37C, 25C
NEB reformulated their buffers.
Buffer 2.1 = old buffer 2 + BSA
Average is more than 90% for PCR-case studies, ApE PCR primer analysis, BLAST assignment.
9-10:15 am, students start to work on master mix,
One student asked why we use XbaI-ApaI to cut plasmid DNA, but use different enzyme to cut PCR fragments.
Problem: it looks students are confused by 20X BSA ad hoc method. I should add BSA directly into the master mix.
I reviewed 2014 midterm exam during the lab period, while the lab instructor worked with the students.
by 2:50pm. Several groups finished experiments. I ask them to finish online notes for lab 6, and the work on 2014 midterm exam.
In class mutant analysis