Sunday, January 11, 2015

564 RLS data from Kaeberlein 05 Science

The 2005 564 RLS data were provided both in Kaeberlein05Science and Managbanag08PlosOne.

In K05, genes were separated into LL, NLL, and SL.  LL null mutants extend RLS >=30%. Putatively, LL RLS <= 20, and LL RLS >=36.  Experiments were repeated until classification can be established. In K05, LL strains can extend RLS with Wilcoxon rank-sum test p-value <0.1, and NSE mutants are those with RLS longer than WT but with p-value >0.1


Byte:Kaeberlein05Science hqin$ cat confirmed.LL.txt 
YNR051C BRE5
YDR110W FOB1 
YOR136W IDH2
YBR267W REI1
YLR371W ROM2 
YDL075W RPL31A 
YLR448W RPL6B
YJR066W TOR1
YNL229C URE2
YBR238C 
YBR255W 
YBR266C

There are 13 LL genes in this list, too few for svm training. It seems that I expanded this list using the ratio of mutant/wt RLS. K05 initially identified 44 putative LL, but only 13 were significant.

Q: How are these categories hold in He14 classification? Why did He14 did not use Kaeberlein05 data?

I need to compare Kaberlein05 with He14 classifications.






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