Thursday, January 29, 2015

Gram stain lab


To prepare fresh bacteria, I can only focus on gram positive ones, because age of the gram-negative bacteria would not influence the gram stain outcomes.

Note that Bacillus subtilis can take 2 days of 30C incubation to form colonies.

Crystal violet, 95% EtOH, Gram Iodine, Safranin.

Problems. Some 95EtOH were contaminated by Iodine, and rubber were oxidized and cracked.

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