Mitochondrial has a proteobacterial origin. E coli is in the proteobacteria phylum.
Student asked, do free electron means free radicals?
Answer should be no. Molecules with 'unpaired' valence electrons are 'free radicals'.
Protein carbonylation caused by ROS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821144/
http://www.hindawi.com/journals/jdr/2012/703538/fig1/
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