For 1000 ml
YE 10 g (Difco)
Peptone 10g (Bacto)
Agar 20 g
fill to 900 ml
weight at 1519gram
autoclave, 45min (autocalve a 100ml cylinder )
weigt at 1648gram.
add 50gram water.
20% glucose 100ml (for 2%glucose) (use the autoclaved cylinder)
http://openwetware.org/wiki/YPD
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