Monday, September 8, 2014

YPD plates


For 1000 ml

YE  10 g (Difco)
Peptone 10g (Bacto)
Agar  20 g
fill to 900 ml

weight at 1519gram
autoclave, 45min (autocalve a 100ml cylinder )
weigt at 1648gram. 

add 50gram water.

20% glucose  100ml (for 2%glucose) (use the autoclaved cylinder)

http://openwetware.org/wiki/YPD

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