The strains mostly at the bottom of their vials due to freezer breakdown one time.
So, I let the vials thaw at RT from 4:21pm-4:30pm.
The vials were completely thawed after 10 minutes at room temperature.
I used suspended the cells with filtered tips, and put them a new MLA plate.
previous plate
I put strains on MLA plates. Some strains grew to tiny colonies in 1 day. Others took 4-5 days to form visible colonies.
No comments:
Post a Comment